JaCVAM Activities

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Update on JaCVAM(updated on Dec.25,2015 )

:completion :On going :Reservation or Reservation :to be started
Classification Test method
(Formal names are shown by mouse over.)
Validation study Peer reviews by experts Test guidelines Regulatory
acceptance by,
e.g., JaCVAM
Regulatory
acceptance board
Recommendation
to
government
01Corrosivity test 01Vitrolife-skin

Reconstructed human tissue (developed in Japan; Vitrolife-Skin) cytotoxicity assay

NIHS
JaCVAM
 
2008.6
2008.8
02Phototoxicity 013T3-NRU

In Vitro 3T3 NRU (neutral red uptake)Phototoxicity Test

COLIPA &
EURL ECVAM
NIHS
OECD TG
No.432

 
 
02Yeast-RBC assay

Combination of yeast growth and hemolysis testing as alternative phototoxicity test

NIHS
JaCVAM
 
2010.1
Reservation
01ROS

ROS (reactive oxygen species) assay

NIHS
JaCVAM
ICH
S10

On going
 
03Skin sensitization test 01LLNA

Local lymph node assay (LLNA)

Not done
ICCVAM
OECD TG
N0.429

 
 
02rLLNA

Reduced LLNA (rLLNA)

EURL ECVAM &
ICCVAM
ICCVAM
OECD TG
N0.429

2012.7
2012.12
03LLNA:DA

LLNA:DA (LLNA modified by Daicel based on ATP content)

NIHS&
JSAAE
JaCVAM or
ICCVAM
OECD TG
N0.442A

2012.10
2012.12
04LLNA:BrdU-ELISA

LLNA:BrdU-ELISA

NIHS&
JSAAE
JaCVAM or
ICCVAM
OECD TG
N0.442B

2012.10
2012.12
05h-CLAT

Human cell line activation test (h-CLAT) for alternative skin sensitization assay

NIHS &
EURL ECVAM
ESAC
Draft TG
On going
 
06DPRA

Direct peptide reactivity assay (DPRA) for alternative skin sensitization assay

EURL ECVAM
ESAC
OECD TG
No. 442C
2015.2
2015.3
07KeratinoSense

KeratinoSense assay for alternative skin sensitization assay

 
ESAC
OECD TG
No. 442D
2015.7
2015.10
08IL-8 Luc assay

IL-8 Luc assay for alternative skin sensitization assay

METI&
JSAAE
On going
Draft TG
 
 
04Skin irritation 01LabCyte EPI-MODEL24

Alternative skin irritation test method using Reconstructed human tissue (developed in Japan; LabCyte EPI-MODEL)

NIHS
OECD
OECD TG
N0.439

2013.6
2013.12
02Episkin, EpiDerm, SkinEthic

alternative skin irritation test method using Reconstructed human tissue (Episkin, EpiDerm, SkinEthic)

EURL ECVAM
ESAC
OECD TG
N0.439

2013.3
2013.3
05Skin absorption test 01In vitro

In vitro

 
OECD
OECD TG
N0.428

2013.10
2014.1
06Ocular irritation test 01BCOP

Bovine corneal opacity and permeability (BCOP) test method

EURL ECVAM &
ICCVAM
ESAC &
ICCVAM
OECD TG
N0.437

2013.10
2014.1
02ICE

Isolated chicken eye (ICE) test method

EURL ECVAM &
ICCVAM
ESAC &
ICCVAM
OECD TG
N0.438

2014.10
2015.1
03FL

Fluorescein Leakage Test Method

EURL ECVAM &
ICCVAM
ESAC
OECD TG
N0.460

2012.10
2012.12
04CM

Cytosensor microphysiometer test

EURL ECVAM
ESAC
Draft TG
On going
 
05STE

Cytotoxicity test: short time exposure (STE) test

NIHS &
JSAAE
ICCVAM
OECD TG
No. 491

On going
 
06SIRC-CVS

Cytyotoxicity test: the rabbit cornea-derived cell line (SIRC-CVS) assay

NIHS
On going
 
 
 
07MATREX

Cytyotoxicity test: Reconstructed human tissue (developed in Japan; MATREX)

NIHS
 
 
 
 
08EpiOcular

Reconstructed coreal tissue assay (EpiOcular)

COLIPA &
EURL ECVAM
ESAC
OECD TG
No. 492

On going
 
09Vitrigel-EIT

Collagen gel (Vitrigel)-eye irritation test (EIT)

MAFF
 
 
 
 
10LabCyte Corne-model

LabCyte Corne-model

JSAAE
 
 
 
 
11OECD TG
No.405 (2012)

Testing for eye irritation /corrosion.

 
ICCVAM review
OECD TG
N0.405

2013.10
2014.1
07Genotoxicity test 01in vitro comet assay

In vitro comet assay

NIHS
 
 
 
 
02in vivo comet assay

In vivo comet assay

NIHS
OECD
OECD TG
No.489

 
 
03 Human Skin Model

Human Skin Model

COLIPA &
EURL ECVAM
 
 
 
 
08Acute oral toxicity test 01Cytotoxicity Test
(estimate starting doses for acute oral systemic toxicity tests)

Cytotoxicity Test(estimate starting doses for acute oral systemic toxicity tests)

EURL ECVAM &
ICCVAM
ICCVAM
OECD GD
N0.129

2011.4
2011.6
02Cytotoxicity test
(LD50 ≧ 2000mg/Kg)

Cytotoxicity test (LD50 ≧ 2000mg/Kg)

 
 
EURL ECVAM
recommendation
On going
 
09Endcrine disrupter screening 01ER-STTA
(agonist) (antagonist)

Estrogen receptor-stably transfected transactivation assay (ER-STTA) for the screening of agonistic activitiy of chemicals

METI
OECD
OECD TG
N0.455

On going
 
02BG1Luc ERTA

BG1Luc estrogen receptor transactivation assay (ERTA) for the screeing of agonistic and anti-agonistic activity of chemicals

ICCVAM
ICCVAM
OECD TG
N0.455

2013.10
2014.1
03CCi

CCi MCF-7 assay for the screening of estrogenic and anti-estrogenic activity of chemicals

ICCVAM
 
 
 
 
04MELN

MELN (derived from MCF-7) cell assay for the screening of estrgenic and anti-estrogenic activity of chemicals

EURL ECVAM
 
 
 
 
05AR-Ecoscreen

AR-EcoScreen (the cell line expressing androgen receptor) assay for the screening of androgenic activity of chemicals

METI
On going
Draft TG
 
 
10Embryonic toxicoty scrneeing 01Hand1-Luc

Hand1-Luc Embryonic Stem Cell Test (Hand1-Luc EST)

METI
 
 
 
 
11Metabolism 01CYP induction method

Cytochrome P-450 induction based metabolic-competent model system

EURL ECVAM
ESAC
Draft TG
On going
 
12Cell transformation assay for the screening of carcinogenitic activity of chemicals 01Bhas

Bhas 42 cell transformation assay

NEDO
ESAC
Draft Guidance
On going
 
02 SHE

Syrian hamster embryo (SHE) cell transformation assay

EURL ECVAM
ESAC
Guidance
On going
 
03Balb

BALB/c 3T3 cell transformation assay

EURL ECVAM
ESAC
Suspended
 
 
13Pyrogen screening test 01In vitro PBMC

in vitro pyrogen test method

EURL ECVAM
EURL ECVAM or
ICCVAM
Ph.Eur.
2010.12
Reservation
All abbreviations that have been used in this table are listed in "Abbreviations". PDFでプリントアウトする

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