Japanese Center for the Validation of Alternative Methods
Office : New Testing Method Assessment, Division of Pharmacology,
National Biological Safety Research Center (NBSRC),
National Institute of Health Sciences (NIHS)

JapaneseEnglish Google WWW jacvam.jp

JaCVAM Activities

  1. Home > 
  2. JaCVAM Activities  > 
  3. Update on JaCVAM

Update on JaCVAM(updated on May.29 , 2014 )

:completion :On going :Reservation or Reservation :to be started
Classification Test name
(Click for details)
Validation study Peer reviews by experts OECD guidelines for the testing of chemicals Regulatory acceptance by, e.g., JaCVAM Regulatory acceptance board Recommendation to government
Collaboration
Corrosivity test (1) Reconstructed human tissue (developed in Japan; Vitrolife-Skin) cytotoxicity assay
NIHS JaCVAM   2008 2008
MHLW
JSAAE
Phototoxicity (1) 3T3 NRU (neutral red uptake) phototoxycity test
COLIPA
ECVAM
NIHS OECD TG No.432    
MHLW
JSAAE
(2) Yeast growth inhibition phototoxicity assay and the red blood cell photohemolysis assay
NIHS JaCVAM   2010 Reservation
MHLW
JSAAE
(3) ROS (reactive oxygen species) assay
NIHS JaCVAM ICH    
MHLW
JPMA
Skin sensitization test (1) Local lymph node assay (LLNA)
Not done ICCVAM OECD TG No.429    
ICCVAM
ECVAM
(2) Reduced LLNA (rLLNA)
ECVAM & ICCVAM ICCVAM OECD TG
No.429
2012 201
ICCVAM
ECVAM
JaCVAM
(3) LLNA-DA (modified LLNA of Daicel based on ATP content)
NIHS JaCVAM or ICCVAM OECD TG
No. 442A

2012

2012
MHLW
JSAAE
(4) LLNA:BrdU-ELISA(BrdU uptake murine LLNA)
NIHS JaCVAM or ICCVAM OECD TG
No. 442B
2012 2012
MHLW
JSAAE
(5) Human cell line activation test (h-CLAT) for skin sensitization screening
NIHS &ECVAM ESAC      
MHLW
ECVAM
(6) Direct peptide reactivity assay (DPRA)
ECVAM ESAC Draft TG On going  
ECVAM
(7) KeratinoSense assay
ECVAM ESAC Draft TG On going  
ECVAM
(8) IL-8 Luc assay
METI        
ICATM
Skin irritation test (1) Reconstructed human tissue (EPISKIN) MTT and IL-1 α assay
ECVAM ESAC Canceld    
ECVAM
(2) Reconstructed human tissue (developed in Japan; LabCyte EPI-MODEL) assay
NIHS OECD OECD TG
No.439
2013 2013
MHLW
JSAAE
(3) Reconstructed human tissue (EPISKIN, EpiDerm, SkiEthic) MTT assay
ECVAM ESAC OECD TG No.439 2010 2012 2010 2012
ECVAM
Skin absorption test in vitro
  OECD OECD TG No.428 2013 2014
 
Ocular irritation test (1) Bovine corneal opacity and permeability (BCOP) assay
ECVAM &
ICCVAM
ECVAM &
ICCVAM
OECD TG
No.437
2013 2014
ICCVAM
(2) Isolated chicken eye (ICE) assay
ECVAM &
ICCVAM
ECVAM &
ICCVAM
OECD TG
No.438
2009 On going 2009
ECVAM
(3) Fluorescein leakage test
ECVAM &
ICCVAM
ESAC OECD TG
No.460
2012 2012
ECVAM
(4) Cytosensor microphysiometer test
ECVAM ESAC Draft TG On going  
ECVAM
(5) Cytotoxicity test: short time exposure (STE) test
NIHS ICCVAM Draft TG On going  
MHLW
JSAAE
(6) Cytyotoxicity test: the rabbit cornea-derived cell line (SIRC-CVS) assay
NIHS        
MHLW
ICATM
(7) Cytyotoxicity test: Reconstructed human tissue (developed in Japan; MATREX)
Reservation        
 
(8) Reconstructed coreal tissue assay
COLIPA/
ECVAM
ESAC      
ECVAM
(9) Collagen gel (Vitrigel)-eye irritation test (EIT)
MAFF        
MAFF
ICATM
(10) Eye irritation assay using anesthetics
  ICCVAM review OECD TG No.405 2013 2014
ICCVAM
Genotoxicity test (1) In vitro mammalian cell micronucleus test (chromosome abnormality test)
    OECD TG473, 487 On going  
JEMS
(2) In vitro commet assay
NIHS        
MHLW
JEMS
MMS
ICCVAM
ECVAM
(3) In vivo comet assay
NIHS OECD Draft TG    
MHLW
JEMS
MMS
ICCVAM
ECVAM
(4) Micronucleus test using reconstructed epidermal model
COLIPA/
ECVAM
       
ECVAM
Acute oral toxicity test (1) In vitro assay
ECVAM &
ICCVAM
ICCVAM OECD GD No.129 2011 2011
ICCVAM
ECVAM
Endcrine disrupter screening (1) Estrogen receptor-stably transfected transactivation assay (ER-STTA) for the screening of agonistic activitiy of chemicals
METI OECD OECD TG No.455    
MHLW、METI
(2) ER-STTA for the screening of anti-agonistic activity of chemicals
METI&MHLW        
MHLW
METI
MFDS
(3) BG1Luc estrogen receptor transactivation assay (ERTA) for the screeing of agonistic and anti-agonistic activity of chemicals
ICCVAM ICCVAM OECD TG
No.457
2013 2014
MHLW
NICEATM / ICCVAM
ECVAM , JaCVAM
(4) CCi MCF-7 assay for the screening of estrogenic and anti-estrogenic activity of chemicals
ICCVAM Reservation      
ICCVAM
KoCVAM
JaCVAM
(5) MELN (derived from MCF-7) cell assay for the screening of estrgenic and anti-estrogenic activity of chemicals
ECVAM        
ECVAM
(6) AR-EcoScreen (the cell line expressing androgen receptor) assay for the screening of androgenic activity of chemicals
METI        
METI
Embryonic toxicoty scrneeing (1)Hand1-Luc Embryonic Stem Cell Test (Hand1-Luc EST)
METI        
NEDO、METI
ICATM
Metabolism (1)Cytochrome P-450 induction-based metabolic-competent model system
ECVAM ESAC      
ECVAM
Cell transformation assay for the screening of carcinogenitic activity of chemicals (1) Bhas 42 cell transformation assay
NEDO ESAC Draft TG On going  
NEDO、METI
(2) Syrian hamster embryo (SHE) cell transformation assay
ECVAM ESAC Draft TG On going  
ECVAM
(3) BALB/c 3T3 cell transformation assay
ECVAM ESAC Reservation    
ECVAN
Pyrogen screening test (1) In vitro assay
ECVAM ECVAM or ICCVAM European Pharmacopoeia 2010 Reservation
ECVAM、ICCVAM

※機関名略称について
abbreviated word official name
COLIPAEuropean Cosmetic,Toiletry and Perfumery Association
ECVAMEuropean Center for the Validation of Alternative Methods
ESACECVAM Scientific Advisory Committee
ICATMInternational Cooperation on Alternative Test Methods
ICCVAMInteragency Coordinating Committee on the Validation of Alternative Methods
JaCVAMJapanese Center for the Validation of Alternative Methods
JEMSThe Japanese Environmental Mutagen Society
JPMAJapan Pharmaceutical Manufacturers Association
JSAAEJapanese Society for Alternatives to Animal Experiments
KoCVAMKorean Center for the Validation of Alternative Method
MAFFThe Ministry of Agriculture, Forestry and Fisheries of Japan
METIMinistry of Economy, Trade and Industry
MFDSMinistry of Food and Drug Safety
MHLWMinistry of Health, Labour and Welfare
NEDONew Energy and Industrial Technology Development Organization
NICEATMInteragency Center for the Evaluation of Alternative Toxicological Methods
NIHS National Institute of Health Sciences
OECDOrganisation for Economic Co-operation and Development

topback
Copyright (C) National Institute of Health Sciences ALL RIGHTS RESERVED.