Japanese Center for the Validation of Alternative Methods
Office : New Testing Method Assessment, Division of Pharmacology,
National Biological Safety Research Center (NBSRC),
National Institute of Health Sciences (NIHS)

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JaCVAM Activities

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Update on JaCVAM(updated on Jan.27 , 2014 )

:completion :on going :reservation or suspended :to be started
Classification Test name
(Click for details)
Validation study Peer reviews by experts OECD guidelines for the testing of chemicals Regulatory acceptance by, e.g., JaCVAM Regulatory acceptance board Recommendation to government
Collaboration
Corrosivity test (1) Reconstructed human tissue (developed in Japan; Vitrolife-Skin) cytotoxicity assay
NIHS JaCVAM   Jun-08 Aug-08
Grants-in-Aid for Scientific Research from MHLW, JSAAE
Phototoxicity (1) 3T3 NRU (neutral red uptake) phototoxycity test
COLIPA and ECVAM NIHS OECD TG No.432    
Grants-in-Aid for Scientific Research from MHLW, JSAAE
(2) Yeast growth inhibition phototoxicity assay and the red blood cell photohemolysis assay
NIHS JaCVAM 2010 Reservation
Grants-in-Aid for Scientific Research from MHLW, JSAAE
(3) ROS (reactive oxygen species) assay
NIHS JaCVAM ICH    
Grants-in-Aid for Scientific Research from MHLW, JPMA
Skin sensitization test (1) Local lymph node assay (LLNA)
Not done ICCVAM OECD TG No. 429    
ICCVAM, ECVAM
(2) Reduced LLNA (rLLNA)
ECVAM and ICCVAM ICCVAM OECD TG No. 429 2012 2012
ICCVAM、ECVAM, JaCVAM
(3) LLNA-DA (modified LLNA of Daicel based on ATP content)
NIHS JaCVAM or ICCVAM OECD TG No. 442A 2012 2012
Grants-in-Aid for Scientific Research from MHLW, JSAAE
(4) LLNA:BrdU-ELISA(BrdU uptake murine LLNA)
NIHS JaCVAM or ICCVAM OECD TG No. 442B 2012 2012
Grants-in-Aid for Scientific Research from MHLW, JSAAE
(5) Human cell line activation test (h-CLAT) for skin sensitization screening
NIHS and ECVAM ESAC      
Grants-in-Aid for Scientific Research from MHLW, ECVAM
(6) Direct peptide reactivity assay (DPRA)
ECVAM ESAC Draft TG on going  
ECVAM
(7) KeratinoSense assay
ECVAM ESAC Draft TG on going  
ECVAM
(8) IL-8 Luc assay
METI        
ICATM
Skin irritation test (1) Reconstructed human tissue (EPISKIN) MTT and IL-1 α assay
ECVAM ESAC Canceld    
ECVAM
(2) Reconstructed human tissue (developed in Japan; LabCyte EPI-MODEL) assay
NIHS OECD OECD TG No. 439 2013 2013
Grants-in-Aid for Scientific Research from MHLW, JSAAE
(3) Reconstructed human tissue (EPISKIN, EpiDerm, SkiEthic) MTT assay
ECVAM ESAC OECD TG No. 439 2010 2012 2010 2012
ECVAM
Skin absorption test In vitro
  OECD OECD TG No. 428 2013 2014
 
Ocular irritation test (1) Bovine corneal opacity and permeability (BCOP) assay
ECVAM and ICCVAM ECVAM and ICCVAM OECD TG No. 437 2009 2013 2009 2014
     
ICCVAM
(2) Isolated chicken eye (ICE) assay
ECVAM and ICCVAM ECVAM and ICCVAM OECD TG No. 438 2009 on going 2009
  
ECVAM
(3) Fluorescein leakage test
ECVAM and ICCVAM ESAC OECD TG No. 460 2012 2012
ECVAM
(4) Cytosensor microphysiometer test
ECVAM ESAC Draft TG On going  
ECVAM
(5) Cytotoxicity test: short time exposure (STE) test
NIHS ICCVAM Draft TG On going  
Grants-in-Aid for Scientific Research from MHLW, JSAAE
(6) Cytyotoxicity test: the rabbit cornea-derived cell line (SIRC-CVS) assay
NIHS        
Grants-in-Aid for Scientific Research from MHLW
ICATM
(7) Cytyotoxicity test: Reconstructed human tissue (developed in Japan; MATREX)
Reservation        
 
(8) Reconstructed coreal tissue assay
COLIPA / ECVAM        
ECVAM
(9) Collagen gel (Vitrigel)-eye irritation test (EIT)
MAFF        
MAFF
ICATM
(10) Eye irritation assay using anesthetics
  ICCVAM review OECD TG No. 405 On going  
ICCVAM
Genotoxicity test (1) In vitro mammalian cell micronucleus test (chromosome abnormality test)
    OECD TG No. 473 and 487 2013 2014
JEMS
(2) In vitro commet assay
NIHS        
Grants-in-Aid for Scientific Research from MHLW,JEMS, MMS, ICCVAM, ECVAM
(3) In vivo comet assay
NIHS OECD Draft TG    
Grants-in-Aid for Scientific Research from MHLW,JEMS, MMS, ICCVAM, ECVAM
(4) Micronucleus test using reconstructed epidermal model
COLIPA / ECVAM        
ECVAM
Acute oral toxicity test (1) In vitro assay
ECVAM & ICCVAM ICCVAM OECD GD No. 129 2011 2011
ICCVAM
ECVAM
Endcrine disrupter screening (1) Estrogen receptor-stably transfected transactivation assay (ER-STTA) for the screening of agonistic activitiy of chemicals
METI OECD OECD TG No. 455    
Grants-in-Aid for Scientific Research from MHLW, METI
(2) ER-STTA for the screening of anti-agonistic activity of chemicals
METI        
Grants-in-Aid for Scientific Research from MHLW, METI, MFDS
(3) BG1Luc estrogen receptor transactivation assay (ERTA) for the screeing of agonistic and anti-agonistic activity of chemicals
ICVAM ICVAM OECD TG No. 457 2013 2014
Grants-in-Aid for Scientific Research from MHLW,ICCVAM, ECVAM, JaCVAM
(4) CCi MCF-7 assay for the screening of estrogenic and anti-estrogenic activity of chemicals
ICCVAM Canceld      
ICCVAM, KoCVAM, JaCVAM
(5) MELN (derived from MCF-7) cell assay for the screening of estrgenic and anti-estrogenic activity of chemicals
ECVAM        
ECVAM
(6) AR-EcoScreen (the cell line expressing androgen receptor) assay for the screening of androgenic activity of chemicals
METI        
METI
Embryonic toxicoty scrneeing (1)Hand1-Luc Embryonic Stem Cell Test (Hand1-Luc EST)
METI        
NEDO
METI
ICATM
Metabolism (1)Cytochrome P-450 induction-based metabolic-competent model system
ECVAM        
ECVAM
Cell transformation assay for the screening of carcinogenitic activity of chemicals (1) Bhas 42 cell transformation assay
NEDO ESAC Draft TG on going  
NEDO
METI
(2) Syrian hamster embryo (SHE) cell transformation assay
ECVAM ESAC Draft TG on going  
ECVAM
(3) BALB/c 3T3 cell transformation assay
ECVAM ESAC Reservation    
ECVAM
Pyrogen screening test (1) In vitro assay
ECVAM ECVAM or ICCVAM European Pharmacopoeia 2010 Reservation
ECVAM
ICCVAM

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